Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Exposure to pollutants is considered one of the potential reasons population declines in bats. In context previous studies, we managed create and keep a wide collection cell lines from European bat species. Liver cells were chosen for testing, as they represent preferred model toxicological studies. Bats are protected, replacing experimental animals thus unique opportunity examine effects which exposed their environments. Moreover, incubation temperature variation may simulate physiological states heterothermic cultivated required number. five different concentrations permethrin (PM) imidacloprid (IMI) used determine cytotoxic these pesticides on Nyctalus noctula -derived liver at 37 °C 8 24 h. An assay based measurement activity lactate dehydrogenase released damaged was quantitating cytotoxicity. Cytotoxicity IMI ranged 0% 47% 56% 67% °C, respectively. PM 36% 43% 88% Permethrin tested an order magnitude lower concentrations; even so, higher degree cytotoxicity recorded. Imidacloprid more toxic hibernation than body °C.